Prebiotic Effect of Oxidized Hydroxypropyl Starch via In Vitro and In Vivo

Most studies on resistant starch are limited to its effect on blood glucose; there are few studies on the prebiotic effects of resistant starch on the gut. In this experiment, through in vivo metabolism verification and in vitro simulated fermentation experiments, it was found that hydroxypropyl oxide (OHS) had a prebiotic effect on the intestine. The results of bioinformatics showed that the structure of the microbiota changed significantly, and the in vitro and in vivo fermentation results of <i>Bacteroides uniformis</i> and <i>Parabacteroides distasonis</i> showed an upward trend. The results of a KEGG prediction of the metabolic pathway showed that Phenylalanine metabolism and Cysteine and methionine metabolism showed an enhanced trend. At the same time, the results of in vitro and in vivo metabolite assays further confirmed this point, and the content of L-Homocystine and Phenylalanine in metabolites decreased significantly, with the decrease in L-Homocystine posing a reduction in cardiovascular disease risk and the decrease in Phenylalanine having a positive significance for phenylketonuria patients. This study proved that hydroxypropyl oxide can regulate the intestinal microbiota and has intestinal prebiotic effects, which can be used to guide the development of functional foods.