Investigation of phenotypic and genotypic characteristics of Acinetobacter baumannii isolated from clinical samples

Objective: Acinetobacter baumannii strains are common nosocomial pathogens worldwide.Our study aimed to determine the antibiotic resistance rate, bla OXA and ISAba1 genes, metallo-beta-lactamases production, biofilm formation, and clonal classification of A. baumannii isolated from clinical samples in orum Erol Olok Training and Research Hospital, Trkiye.It was also aimed to describe the plasmid profile and analyze the association between genes, clones, and plasmids.Methods: Ninety-eight A. baumannii isolated between 2018 and 2019 were included in the study.Antibiotic susceptibility tests were determined with Vitek 2. The reference broth microdilution method was used to assess colistin susceptibility.These results were compared with those obtained from the Vitek 2. A multiplex polymerase chain reaction detected bla OXA-23 , bla OXA-24/40 , bla OXA-51 , and bla OXA-58 genes.The ISAba1/ bla OXA-23 and ISAba1/ bla OXA-51 genes were analyzed separately via PCR.Genotypes and subtypes of the isolates were determined with the Repetitive Extragenic Palindromic