Nanofiber based Optical Sensor for Hydrogen Peroxide Using Carbon Quantum Dots

Wounds signify disruptions in the structural integrity of the skin and underlying tissues, resulting from a range of factors including trauma, surgical procedures, or pathological conditions. These wounds can be further complicated by underlying medical issues and insufficient tissue oxygenation. However, wound healing is a multifaceted and dynamic process aimed at restoring the structure and function of damaged tissues, influenced by a combination of intrinsic and extrinsic factors. Within this intricate process, reactive oxygen species (ROS) play a crucial role, acting as key regulators in the body's response to facilitate wound repair. In this study, a sensor is developed by dispersing carbon quantum dots (CQD) in a polyvinyl alcohol (PVA) solution. CQD displays excellent biocompatibility and photoluminescence properties, rendering them highly suitable for hydrogen peroxide detection. These probes exhibit remarkable sensitivity and selectivity toward hydrogen peroxide, enabling accurate detection even at low concentrations. By utilizing electrospinning methods, CQD-PVA nanofibers are fabricated, and their morphology is analyzed using scanning electron microscopy and goniometer assessments. Furthermore, the accuracy of the sensor is evaluated using a biomimetic fluid, and fluorescence spectroscopy is employed to quantitatively estimate H<inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf>O<inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> levels, which directly reflect the progress of wound healing.