Influence of Ionizing Radiation on Bio-synthesis of Noble Metal SF-AgNPs: Their Applications

Objectives: The aim of this study was to explore the rapid and extracellular synthesis of silver nanoparticles (AgNPs) using aqueous Bombyx Mori Silk fibroin (SF) solution, facilitated by UV irradiation. The study seeks to assess the stability, size, shape, and other key properties of the synthesized silver nanoparticles. Methods: Silver nanoparticles were synthesized by exposing the silk fibroin solution to UV radiation characterization of the synthesized AgNPs was carried out using various techniques, including UV-Vis spectroscopy to confirm nanoparticle formation, X-ray diffraction (XRD) for structural analysis, transmission electron microscopy (TEM) for morphological observation, Fourier transform infrared spectroscopy (FT-IR) to identify protein functional groups, and thermo gravimetric analysis (TGA) and differential scanning calorimetry (DSC) to assess thermal stability. Findings: UV-Vis spectra confirmed the formation of silver nanoparticles, with an increase in absorption corresponding to longer irradiation times. XRD analysis revealed a face centered cubic structure, indicating metallic silver nanoparticles. TEM images showed the nanoparticles to be spherical or nearly spherical in shape. FT-IR spectra indicated the involvement of silk fibroin proteins in the synthesis process. The TGA and DSC results demonstrated enhanced stability of the SF/AgNPs composites compared to bulk materials. Novelty: This study presents a simple, eco-friendly biosynthesis approach for silver nanoparticles using Bombyx mori silk fibroin and UV irradiation. The method provides a controlled synthesis process, allowing for the adjustment of particle size and shape through irradiation parameters, offering an alternative to more traditional physical and chemical methods in nanoparticle synthesis. The stability and minimal chemical reactivity of the synthesized nanoparticles enhance their potential applications in various fields, including medicine, environmental protection, and nanotechnology. Keywords: Bombyx mori silk, Silver nanoparticles, UV-Vis, TEM, TGA, DSC