Occurrence of Rubus yellow net virus in red raspberry orchards in Serbia

A survey for Rubus yellow net virus (RYNV) in red raspberry orchards in Serbia was conducted from 2014‒2022. A total of 112 samples from seven cultivars were collected and analyzed. Reverse transcription polymerase chain reaction (RT-PCR) using RYNV-specific primers was used for virus detection. A specific 350 bp fragment was obtained in 30 samples, confirming RYNV presence in cultivars ‘Fertödi Zamatos’, ‘Meeker’, ‘Tulameen’, and ‘Willamette’. Sequence analysis of the partial polyprotein gene of the RYNV genome confirmed that selected Serbian isolates share 83.43–98.29% nucleotide identities with RYNV sequences in GenBank.