Complete Mitochondrial Genome of Calanoid Copepod Bestiolina similis (Sewell, 1914)

This study sequenced the mitochondrial genome of the copepod Bestiolina similis. DNA was extracted using a Qiagen Kit, and library preparation, PCR amplification, and sequencing were performed. The mitochondrial genome was 23,704 base pairs long, consisting of 13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNA genes. Its nucleotide composition is 43.42% adenine, 43.22% thymine, 6.48% guanine, and 6.88% cytosine. Phylogenetic analysis shows B. similis as a relict species, closely related to Labidocera rotunda and Calanus simillimus, with an independent evolutionary divergence from other copepods.