Bioinformatics Analysis: COL1A1 May Be a Key Target for Helicobacter Pylori to Participate in the Process of Gastric Adenocarcinoma through Cytotoxin-Associated Protein A

目的：幽门螺杆菌细胞毒素相关蛋白A是一种已知的可诱导胃癌发展的重要毒力因子。本研究旨在通过生物信息学进一步确定具体在这一过程中发挥作用的生物学靶点。材料方法：首先，从基因表达综合数据库中筛选mRNA表达谱数据集GSE29998，并借助已有的研究成果，使用R软件(4.0.0版)分析幽门螺旋杆菌通过细胞毒素相关蛋白A参与胃腺癌进程的潜在差异表达基因，再借助富集分析进一步阐述其意义。并最终根据蛋白质互作网络的hub基因的排名，筛选出可能的潜在靶向基因。结果：我们通过差异性分析确定了540个可能的与胃腺癌相关的潜在差异基因。再结合陈等人的研究成果所提供的基因数据库，我们进一步确认18个与潜在的幽门螺杆菌细胞毒素相关蛋白A相关的胃腺癌差异表达基因。蛋白质–蛋白质相互作用网络提示只有COL1A1处于枢纽位置。因此我们认定COL1A1是与幽门螺杆菌细胞毒素相关蛋白A相关的胃腺癌差异表达基因。结论：我们的研究鉴定了在胃腺癌中18个潜在的幽门螺杆菌细胞毒素相关蛋白A相关的差异表达基因。幽门螺杆菌细胞毒素相关蛋白A可能借助COL1A1参与胃腺癌的发生发展过程中。本研究结果首次借助细胞实验相关数据筛选出临床样本中幽门螺杆菌可能影响胃腺癌发生的潜在生物学靶点，这可能有助于我们进一步理解这一疾病。Introduction: Helicobacter pylori cytotoxin-associated protein A is an important virulence factor known to induce the development of gastric cancer. This research aims to further determine the specific biological targets that play a role in this process through bioinformatics. Material method: Firstly, the mRNA expression profile dataset GSE29998 was screened from the comprehensive database of gene expression. With the help of existing research results, the potential differentially expressed genes of Helicobacter pylori involved in the process of gastric adenocarcinoma through cytotoxin-associated protein A were analyzed using R software (version 4.0.0), and its significance was further elaborated with the help of enrichment analysis. And finally, based on the ranking of hub genes in the protein-protein interaction network, possible potential targeted genes are screened out. Result: Through differential analysis, we identified 540 possible potentials differentially expressed genes related to gastric adenocarcinoma. Combined with the gene database provided by the research results of Chen et al., we further confirmed 18 differentially expressed genes of gastric adenocarcinoma related to the potential Helicobacter pylori cytotoxin-associated protein A. The protein-protein interaction network suggests that only COL1A1 is in the hub position. Therefore, we determined that COL1A1 is A differentially expressed gene in gastric adenocarcinoma associated with Helicobacter pylori cytotoxin-associated protein A. Conclusion: Our research identified 18 potentials differentially expressed genes related to Helicobacter pylori cytotoxin-associated protein A in gastric adenocarcinoma. Helicobacter pylori cytotoxin-associated protein A may be involved in the occurrence and development of gastric adenocarcinoma with the help of COL1A1. The results of this study are the first to screen out potential biological targets that Helicobacter pylori may affect the occurrence of gastric adenocarcinoma in clinical samples with the help of relevant data from cell experiments, which may help us further understand this disease.