Polyoxysteroids of plants of the genus Silene L. and their derivatives as potential anti-inflammatory agents

According to literature and our experimental data, plant polyoxysteroids or phytoecdysteroids are found in more than 126 species of Silene L. growing in Kazakhstan (12 endemic species). In the last decade, scientific interest in polyoxysteroids as substances with potentially pharmacologically active properties has led to an increase in the number of studies aimed at their isolation, quantitative determination and chemical modification. Objective. Study the anti-inflammatory activity of new derivatives of polyoxysteroids of the genus Silene L. in vivo conditions.Methods. The studies were conducted by the method of supramolecular complexation and mechanochemistry, determination of transmembrane permeability and water solubility. Results. To obtain new water-soluble derivatives with increased bioavailability, solid dispersions and complexes of 20-hydroxyecdysone (20E) with disodium salt of glycyrrhizic acid (Na₂GA) and 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD) were prepared using mechanochemical methods. Several supramolecular encapsulated complexes based on polyoxysteroids were also synthesized, and their water solubility in different ratios and anti-inflammatory activity at doses of 25 mg/kg and 50 mg/kg were studied. The supramolecular β-cyclodextrin complex of 3-epi-2-deoxyecdysone demonstrated significantly greater anti-inflammatory activity at both doses than sodium diclofenac.Conclusion. The results of the study showed that new supramolecular complexes of polyoxysteroids with α-, β-, γ- and 2-hydroxypropyl – β – cyclodextrins and disodium salt of glycyrrhizic acid and mechanochemically obtained solid dispersions can be recommended for use in the development of an anti-inflammatory herbal medicine origin, due to their high anti-inflammatory effectiveness.Keywords: genus Silene L., polyoxysteroids, supramolecular complexes, mechanocomposites, anti-inflammatory activity.