COMPARISON OF THE EFFECTIVENESS OF NON-THERMAL METHODS FOR ENSURING MICROBIOLOGICAL SAFETY OF FOOD WASTE

Ensuring microbiological safety and extending shelf life, combined with the preser vation of thermolabile substances contained in food products, necessitate the search for effective non-thermal methods of food processing. This study shows a comparison and quantitative justifi cation of the effectiveness of various methods of non-thermal exposure to milk containing E. Coli M-17 E. coli as a method of ensuring microbiological safety. To simulate contamination, an aque ous solution of dry lyophilizate of live bacteria of E. Coli M-17 strain “Colibacterin” with the concentration of the preparation not less than 1 g/liter of water was introduced into a 200 ml por tion of milk. The crops were carried out on a nutrient medium for the isolation of enterobacteria “AGAR ENDO-GRAM”. Exposure of milk samples was performed in laboratory stands for 60 minutes. It was found that combined exposure to ozone with a capacity of 400 mg/h and ultravio let waves with a wavelength of 260 nm allows to achieve total suppression of colony development of E. Coli M-17 in milk with a duration of exposure of only 15 minutes. Using exposure to ozone or UV wavelengths separately from each other for 60 minutes allows to suppress more than 98 % of E. Coli M-17 colonies in milk (21 CFU and 273 CFU respectively against 18717 CFU of con trol sample). Exposure to a combination of 400 mg/h ozone and 1.7 MHz ultrasonic waves for 30 minutes suppresses the development of all E. Coli M-17 colonies in milk. Exposure of the tested sample to electromagnetic and electrostatic fields separately, as well as their combination, showed low efficiency in suppressing the development of E. Coli M-17 colonies in milk.

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Publication Details

Journal
Bulletin of the South Ural State University Series Food and Biotechnology
Published
2025-01-01
DOI
https://doi.org/10.14529/food250209
Primary Topic
Food Industry and Aquatic Biology
Type
article
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article

COMPARISON OF THE EFFECTIVENESS OF NON-THERMAL METHODS FOR ENSURING MICROBIOLOGICAL SAFETY OF FOOD WASTE

А. Л. Кузнецов, O.A. Suvorov, Nikolai E. Vorotyntsev, Elina A. Bazankova
Bulletin of the South Ural State University Series Food and Biotechnology
Food Industry and Aquatic Biology
article

COMPARISON OF THE EFFECTIVENESS OF NON-THERMAL METHODS FOR ENSURING MICROBIOLOGICAL SAFETY OF FOOD WASTE

А. Л. Кузнецов, O.A. Suvorov, Nikolai E. Vorotyntsev, Elina A. Bazankova
article en

Abstract

Ensuring microbiological safety and extending shelf life, combined with the preser vation of thermolabile substances contained in food products, necessitate the search for effective non-thermal methods of food processing. This study shows a comparison and quantitative justifi cation of the effectiveness of various methods of non-thermal exposure to milk containing E. Coli M-17 E. coli as a method of ensuring microbiological safety. To simulate contamination, an aque ous solution of dry lyophilizate of live bacteria of E. Coli M-17 strain “Colibacterin” with the concentration of the preparation not less than 1 g/liter of water was introduced into a 200 ml por tion of milk. The crops were carried out on a nutrient medium for the isolation of enterobacteria “AGAR ENDO-GRAM”. Exposure of milk samples was performed in laboratory stands for 60 minutes. It was found that combined exposure to ozone with a capacity of 400 mg/h and ultravio let waves with a wavelength of 260 nm allows to achieve total suppression of colony development of E. Coli M-17 in milk with a duration of exposure of only 15 minutes. Using exposure to ozone or UV wavelengths separately from each other for 60 minutes allows to suppress more than 98 % of E. Coli M-17 colonies in milk (21 CFU and 273 CFU respectively against 18717 CFU of con trol sample). Exposure to a combination of 400 mg/h ozone and 1.7 MHz ultrasonic waves for 30 minutes suppresses the development of all E. Coli M-17 colonies in milk. Exposure of the tested sample to electromagnetic and electrostatic fields separately, as well as their combination, showed low efficiency in suppressing the development of E. Coli M-17 colonies in milk.

Bulletin of the South Ural State University Series Food and BiotechnologyVol. 13(2)
Responsible consumption and production
Openalex Percentile: Top 83%
Food Industry and Aquatic Biology
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