Ultrasensitive detection of deoxynivalenol by mass spectrometric immunoassay with cascade signal amplification
We developed a novel mass spectrometry immunoassay for the ultrasensitive and selective detection of deoxynivalenol by introducing nucleic-acid-amplification techniques. By selecting thymine as a mass tag, the primer DNA modified on the antibody could serve dual functions as both a signal probe and an initiator for the cascade amplification reaction.
Authors
- Hanxu Ji (ORCID: https://orcid.org/0000-0003-0401-6985)
- Jingjing Xu (ORCID: https://orcid.org/0000-0002-1050-7660)
- Diyao Jiang
- Yuwei Qiang
- Xiuming Mei
- Xiaoxiao Wu (ORCID: https://orcid.org/0000-0002-1515-9546)
- Yufeng Li (ORCID: https://orcid.org/0000-0002-5013-5849)
- Chi Zhang (ORCID: https://orcid.org/0000-0002-7511-805X)
- Jungui Zhou
- Miao Yang
Institutions
- Nanjing Product Quality Supervision and Inspection Institute (CN)
Publication Details
- Journal
- Analytical Methods
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5ay00724k
- Primary Topic
- Mycotoxins in Agriculture and Food
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Science and Technology Support Program of Jiangsu Province
- National Key Research and Development Program of China