Isolation, Characterization and quantification of gallic acid from hydro alcoholic flower extract of Woodfordia fruticosa (L.) kurz along with fingerprinting analysis by HPTLC, HPLC, FT-IR, and NMR (1 H and 13C) spectroscopy

Ethnopharmacological knowledge of the plant Woodfordia fruticosa (L.) Kurz. is very precious in traditional system of medicine. Gallic Acid (3,4,5-trihydroxybenzoic acid) is one of the most abundant phenolic acids of the flower extract of this plant. Gallic acid (GA) shows a broad range of beneficial effects in prevention and treatment of several diseases. The pharmacological activities of GA are antioxidant, anti-diabetic, antimicrobial, anti-inflammatory, cardioprotective, anticancer, neuroprotective and gastroprotective. The research deals with isolation and quantification of GA by using Column Chromatography and HPLC. The abundant of Gallic acid (GA) was come out 2.28µg/mg in the WFF extract. The FT-IR spectrum analysed the presence of carboxylic group (2700-3600 cm-1), hydroxyl phenolic groups (3284, 3382 cm-1), carbonyl group (1705 cm-1) and an aromatic moiety (1541, 1618 cm-1). 1H-NMR and 13C- NMR spectrum of Gallic Acid (GA) was successfully recorded. So the hydro alcholic extract of WFF confirm the presence of Gallic Acid.