A novel pyrrolidine alkaloid and a novel isoflavone with anti-inflammatory activity from the tubers of <i>Arisaema franchetianum</i>

Solanpyrrole B (<b>1</b>), a new pyrrolidine alkaloid and 5,7,4',5'-tetrahydroxy-6, 3'-dimethoxyisoflavone (<b>2</b>), a new isoflavone, along with 12 known compounds (<b>3</b>-<b>14</b>), were isolated from the tubers of <i>Arisaema franchetianum</i>. The chemical structures of these compounds were identified by using spectroscopic analysis (NMR, UV, IR, HR-ESI-MS). Compounds <b>1</b> and <b>2</b> (10 <i>μ</i>M) exhibited anti-inflammatory activities in inhibiting the NF-κB signaling pathway, reducing the release of NO from RAW264.7 macrophages and BV2 microglia induced by LPS. Compounds <b>1</b>, <b>2</b> and <b>11</b> exhibited significant cytotoxic activity against HCT116 cells (IC₅₀ = 9.16 ± 1.59 <i>μ</i>M), HGC27 cells (IC₅₀ = 7.98 ± 1.07 <i>μ</i>M), U87MG (IC₅₀ = 2.81 ± 0.18 <i>μ</i>M), respectively.