Growth-independent CLA production by Bifidobacterium breve JKL2022 and the potential transcriptional regulatory role of TetR in linoleic acid isomerase expression

The ability of B. breve JKL2022 strain to convert free LA to CLA during growth, as well as using washed cells and crude protein extracts, suggests strain specificity and superior enzymatic activity. In addition to its potential application as a probiotic strain with CLA-enhancing properties, washed JKL2022 cells or crude protein extracts can be developed as postbiotic preparations for the same purpose.