Multiplex droplet digital PCR for the detection and quantitation of Streptococcus pneumoniae, Mycoplasma pneumoniae, and Haemophilus influenzae
The multiplex ddPCR assay established in this study can accurately detect <i>S. pneumoniae</i>, <i>M. pneumoniae</i> and <i>H. influenzae</i> DNA and can be used as an auxiliary tool for the clinical identification of pathogens and guidance of antibiotic therapy.
Authors
- Yaling Cao (ORCID: https://orcid.org/0000-0002-4952-0643)
- Jing Huang (ORCID: https://orcid.org/0000-0003-2253-0507)
- Ling Xu (ORCID: https://orcid.org/0000-0002-7044-7663)
- Zihao Fan (ORCID: https://orcid.org/0000-0003-0011-8394)
- Xinping Zhu (ORCID: https://orcid.org/0000-0003-0460-3514)
- Junwen Wang (ORCID: https://orcid.org/0000-0002-4432-4707)
- Feng Ren (ORCID: https://orcid.org/0000-0003-3128-1434)
- Yitong Jiang
- Yuan Tian (ORCID: https://orcid.org/0009-0006-3773-8131)
- Xiangying Zhang (ORCID: https://orcid.org/0000-0003-3662-4491)
- Yinkang Mo
- Zhenzhen Pan
Institutions
- Capital Medical University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Beijing Chao-Yang Hospital, Capital Medical University (CN)
- Peking Union Medical College Hospital (CN)
- Beijing YouAn Hospital (CN)
- Beijing Chuiyangliu Hospital (CN)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-06-20
- DOI
- https://doi.org/10.3389/fcimb.2025.1548492
- Citations
- 3
- Primary Topic
- Pneumonia and Respiratory Infections
- Type
- article
- Field-Weighted Citation Impact
- 4.06
Funders
- National Natural Science Foundation of China
- Capital Medical University