A Novel UV-VIS Spectroscopic Approach for the Development and Validation of Benzoyl Peroxide Quantification Methods

Objective: Benzoyl peroxide (BPO) is a widely used active ingredient in acne treatments, recognized for its antibacterial and exfoliating properties. This study aims to quantitatively determine the concentration of BPO in an acne cream using UV-Vis spectrophotometry. Methods: the method involves dissolving the cream in a suitable solvent and measuring its absorbance at a specific wavelength. The UV-Vis technique has demonstrated accuracy and reliability quantifying BPO in commercial formulations. This method is suitable for use in quality control and formulation standardization in the pharmaceutical and cosmetic industries. Results: the UV-Vis method was used to determine the concentration of BPO in the product. This quantitative analytical method was validated according to ICH guidelines at a wavelength of 235nm, range of determination is 4.8 µg/ml to 7.2 µg/ml. Conclusion: the proposed method for analyzing BPO content is simple, highly reliable and well-suited for use in laboratory settings.