A draft genome assembly for the dart-poison frog Phyllobates terribilis
Dendrobatid poison frogs have become well established as model systems in several fields of biology. Nevertheless, the development of molecular and genetic resources for these frogs has been hindered by their large, highly repetitive genomes, which have proven difficult to assemble. Here we present a draft assembly for <i>Phyllobates terribilis</i> (12.6 Gb), generated using a combination of sequencing platforms and bioinformatic approaches. Similar to other poison frog sequencing efforts, we recovered a highly fragmented assembly, likely due to the genome's large size and very high repeat content, which we estimated to be ≍88%. Despite the assembly's low contiguity, we were able to annotate multiple members of three gene sets of interest (voltage-gated sodium channels and <i>Notch</i> and <i>Wnt</i> signaling pathways), demonstrating the usefulness of our assembly to the amphibian research community.
Authors
- Kerry L. Gendreau (ORCID: https://orcid.org/0000-0001-6058-0893)
- Daniel Janies (ORCID: https://orcid.org/0000-0002-7890-9906)
- Ralph A. Saporito (ORCID: https://orcid.org/0000-0003-4762-2774)
- Marcus R. Kronforst (ORCID: https://orcid.org/0000-0002-9825-891X)
- Taran Grant (ORCID: https://orcid.org/0000-0003-1726-999X)
- Denis Jacob Machado (ORCID: https://orcid.org/0000-0001-9858-4515)
- Roberto Márquez (ORCID: https://orcid.org/0000-0002-0644-3078)
- Reza Nouri (ORCID: https://orcid.org/0000-0002-7368-7064)
Institutions
- University of North Carolina at Charlotte (US)
- Universidade de São Paulo (BR)
- John Carroll University (US)
- University of Chicago (US)
- Virginia Tech (US)
Publication Details
- Journal
- Gigabyte
- Published
- 2025-06-20
- DOI
- https://doi.org/10.46471/gigabyte.157
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- University of Chicago
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- University of North Carolina at Charlotte
- National Institute of General Medical Sciences