Green synthesized Silver Nanoparticles using leaf extract of Nicotiana rustica Natural Pesticides against Indian white termites, Odontotermes obesus

– Green nanotechnology has widespread applications in various biomedical science fields. In this research paper, green-synthesized silver Nanoparticles, prepared by using Nicotiana rustica extract, were characterized using digital calorimeter, scanning electron microscopy and FTIR. This study thoroughly investigated the insecticidal efficacy of medicinal plants, Nicotiana rustica. Different extraction methods were used and their efficacy with and without nanoparticles was compared. FT-IR analysis indicated the involvement of carboxyl (-C=O), hydroxyl (-OH), C-N stretching of aromatic amines or NO2 symmetric stretching and amine (-NH) functional groups of tobacco leaf extract in the preparation of Silver Nanoparticles. SEM analysis showed that AgNPs is widely distributed in some areas and has irregular and circular shapes that have different diameters with average diameters of, for example, 54.79 nm and 66.05 nm. The focus of the study was on investigating various parameters such as mortality rates, insect survival rates, adult emergence and development stages after treatment periods of 24, 42 and 72 hours. The termite repellent efficacy was significantly higher in treatments with nanoparticles, but the differences were not significant (p>0.05).