Physicochemical properties of micromycetes Alternaria alternate 4 lectin

The physicochemical properties of the lectin of the lower fungus, micromycetes Alternaria alternate 4 have been studied. It was found that the lectin of the micromycetes Alternaria alternate 4 is stable over a wide range of temperatures and pH values (5-70oC and pH 6.0-9.0). The greatest activity of lectin was observed at a temperature of 5-50 °C and a pH of 6.5-8.5. The increased activity of Alternaria alternate 4 lectin was facilitated by the presence of divalent Ca2+, Mn2+, and Zn2+ ions in the reaction mixture, which indicates that Alternaria alternate 4 lectin is a metal-dependent glycoprotein. Alternaria alternate 4 lectin had the ability to bind simple carbohydrates. The activity of Alternaria alternate 4 lectin was inhibited to a greater extent by galactose, which indicates that Alternaria alternate 4 lectin belongs to the group of galactose-binding lectins.