Development and Validation of Q-Absorbance Ratio Spectrophotometric Method for the Simultaneous Estimation of Lamivudine and Zidovudine in bulk and formulation.

The present research work demonstrates an analytical method development for simultaneous estimation of Lamivudine and Zidovudine in combined dosage form using Q-absorbance ratio concept. While method development, two different wavelengths one representing iso-absorptive point (271 nm) and other representing the λmax of Lamivudine (269 nm) were used. Optimum response was obtained in solvent system that comprises methanol and water in ratio of 60:40 v/v. Proposed UV method was found to be linear over the concentration range of 1-20 µg/ml for Lamivudine and that of 1-16 µg/ml for Zidovudine.