An SNP-based diagnostic method for Brucella S2 vaccine strain infections
SNP-based ddPCR methods demonstrate higher sensitivity than qPCR methods and enable specific detection of brucellosis caused by the S2 vaccine strain. Reduction of probe overlap with the transient stem-loop structure improves detection sensitivity, providing valuable insights for enhanced PCR amplification efficiency.
Authors
- Wanru Geng (ORCID: https://orcid.org/0000-0001-7088-4938)
- Jingbo Zhai (ORCID: https://orcid.org/0000-0001-8891-279X)
- Hui Wang (ORCID: https://orcid.org/0009-0001-8635-033X)
- Xiaowei Tian
- Yan Yang
- Xingya Wang (ORCID: https://orcid.org/0000-0003-0934-4689)
- Wanyang Li (ORCID: https://orcid.org/0000-0002-7113-3789)
- Shuai Zhang (ORCID: https://orcid.org/0000-0003-0094-1233)
Institutions
- Inner Mongolia University for Nationalities (CN)
- Minzu University of China (CN)
- Tongliao Academy of Agricultural Sciences (CN)
- Hulunbuir University (CN)
Publication Details
- Journal
- Frontiers in Veterinary Science
- Published
- 2025-06-19
- DOI
- https://doi.org/10.3389/fvets.2025.1570220
- Citations
- 1
- Primary Topic
- Brucella: diagnosis, epidemiology, treatment
- Type
- article
- Field-Weighted Citation Impact
- 2.35
Funders
- National Natural Science Foundation of China