Development of methods for standardization of a water-soluble sulfur-containing antioxidant sodium 3-(3'-tret-butyl-4'-hydroxyphenyl)propylthiosulfonate (TS-13)

Introduction. Oxidative stress plays a key role in the pathogenesis of various human pathologies, including cardiovascular disease, cancer, neurological disorders, Parkinson’s disease, Alzheimer’s disease, inflammatory diseases, muscular dystrophy, liver diseases, and even human aging. The prevalence of the above diseases is a significant challenge for modern medicine. One of the possible solutions is the development of novel pharmaceutical agents for the prevention and comprehensive treatment of these conditions. Such agents include, in particular, antioxidants. Aim. Development of standardization methods for a sulfur-containing antioxidant sodium 3-(3’-tret-butyl-4’-hydroxyphenyl)propylthiosulfonate (TS-13). Materials and methods. The object of the study was a substance of the water-soluble antioxidant TS-13. The methods of spectrophotometry, voltammetry, titration and thin-layer chromatography were used. Results. The developed methods for the quantitative determination of TS-13 have been validated in accordance with the General Pharmacopeial Monograph 1.1.0012 “Validation of analytical methods”. The correlation coefficient of each method is not less than 0,997, the relative standard deviation is not more than 2%. Conclusion. The results of this study demonstrate the potential for utilizing the proposed techniques in the quality control of a novel water-soluble antioxidant TS-13.