Probiotic and Synbiotic Isolates From Whey Water and Fermented Rice Water Exert Compelling Wound‐Healing Efficacy in a Diabetic Mouse Model

Probiotics serve as essential wound healers, particularly in diabetic patients, where they eliminate the interfering hindrance caused due to enhanced oxidative stress, inflammation, and microbial imbalances. Probiotic strains are also reported to enhance the level of tissue regeneration-specific cytokines and growth factors. Similarly, probiotics also stop infections by preventing the growth of harmful bacteria at the lesion site, which may be helpful for susceptible diabetic ulcers. Thus, probiotics may be a viable adjuvant treatment to hasten the healing of diabetic wounds. The present study aims to evaluate the efficacy of probiotic and synbiotic supplementation on wound healing in diabetic mice. Accordingly, probiotic strains Bacillus cereus strain BWN SC and Bacillus aerophilus strain BWN SC were isolated from whey water and fermented rice water on MRS agar media. Their wound-healing potential was investigated in male Swiss albino mice. Initially, streptozotocin was used to induce diabetes in the animals, after which 3 mm subcutaneous puncture wounds were induced on the dorsal region. Next, they were treated with different probiotics or synbiotics applied topically on the wounded area. Simultaneously, oral supplementation of the respective strains was also done using gavage. Wound contraction rate was studied at intervals of 1, 5, 10, and 14 days, followed by subsequent histological assay and hematological analysis. Animals treated with probiotics and synbiotics supplementation showed a faster wound contraction rate as compared to the control. Hematological analysis also showed improved wound-healing parameters in the treated mice. Thus, isolated probiotic strains can be effectively used for wound healing in diabetic mice. They can be combined with prebiotics to get even more potent therapeutic tool in the form of synbiotics.