Anti-inflammatory Activity of Sprouts according to Hydro-priming and Light Quality Levels in Vigna vexillata (L.) A.Rich. var. tsusimensis Matsum. Seeds

This study was performed to confirm the optimal conditions for the germination and production of high-functional sprouts of Vigna vexillata (L.) A.Rich.var.tsusimensis Matsum.seeds known to be a native, medicinal plant in Korea.The seed length and width were respectively 5.83 mm and 4.08 mm, the weight of 1,000 seeds was 51.08 g, and the seed viability rate was 80.0%.Seed germination was highest at 30C under light conditions and at 25C under dark conditions, and germination tended to be inhibited regardless of the light condition at 15C.It was confirmed that as the germination percentage (GP) increased, T 50 and mean germination time were shortened, with germination rate increasing as the temperature was increased under light conditions.The effect of separation was confirmed by distinguishing absorbed seeds and unabsorbed seeds according to soaking period and temperature.The difference in GP between absorbed seeds (64%) and unabsorbed seeds (15%) was 49%, which had the effect of seed separation.We considered the optimal soaking treatment to be two days at 15C to separate non-dormant seeds and to produce sprouts.When seeds were hydro-primed according to the light quality by applying optimal immersion conditions, GP was excellent in blue light, and sprout growth was excellent in all light quality conditions.Sprout extract had the best antioxidant content and activity under the blue light treatment and had the best NO production inhibition effect under the red light treatment.