Effect of Different Nutrient Media on Mycelial Growth of Different Species of Medicinal Mushroom

The research was carried out in 2022 in the National Commission for Biotechnology (NCBT). The aim is studying the mycelium growth of 5 commercial strains of four medicinal mushroom: Ganoderma lucidum (G.l), Agaricus blazei (A.b), Hericium erianaceum (H.e) and Lentinus edodes (Tow strains: strain M3782 (L.e1) and strain straw (L.e2)), sourced of Aloha company of USA on four nutrient growth media: Potato Dextrose Agar (PDA), Malt extract Agar (MEA), Sabouroud Dextrose Agar (SDA) and Corn meal agar (CMA). The following mycelium growth parameters were studied: mycelium height (H), mycelium diameter (D), mycelium density (G), growth rate (GR) and growth coefficient (GC). Results were analyzed by using XLSTAT 2018, ANOVA analysis and Fisher test. The results showed that all strains differed in all growth parameters on the deferent nutrient media. The strains of G.l, A.b and L.e2 recorded the highest value of the growth coefficient on the MEA medium (35.09, 16.51 and 22.27, respectively) with significant superior than the growth coefficient on the other media. The two strains of H.e and L. e1 had a higher growth coefficient on the media PDA (27.27 and 19.11, respectively), MEA (27.49 and 20.86 respectively) and SDA (24.59 and 21.34 respectively), with a significant difference from the value of the growth coefficient recorded on the media CMA (2.36 and 7.01 respectively). The growth was slow for all strains on all media. On the PDA, MEA and SDA media, the growth was slow for the strain H.e, and very slow for the strain A.b, and differed for the other strains between slow and very slow. The growth on CMA medium of all strains was very slow. The difference in the growth rate was clear. The highest growth rate was for strains G.l, A.b and H.e on the MEA medium (5.54, 1.92 and 1.76 mm/day respectively) with significant differences and for the two strains of L.e on the CMA medium (3mm/day for strain L.e1 and 2.99mm/day for strain L.e2). It is recommended that additional nutrient media to be used for growth and isolation of fungal strains to point out the best media for each strain.