The Role of Genetic Variation in Modulating the Effects of Blended Fruits and Vegetables Versus Fruit- and Vegetable-Coated Food Products on Antioxidant Capacity, DNA Protection, and Vascular Health: A Randomized Controlled Trial

<b>Background/Objectives</b>: Fruits and vegetables (F&Vs) are major dietary sources of phytochemicals, crucial for preventing non-communicable diseases. However, barriers such as preparation inconvenience and a short shelf life hinder their consumption. F&V-coated foods have emerged as an alternative. This human nutrition intervention study assessed the effects of a blended F&Vs mixture versus an F&V-coated food on phytochemical absorption and chronic disease risk markers. It also explored how genetic variation influences physiological responses to these F&V products. <b>Methods</b>: In this randomized-controlled trial, participants were assigned to one of three dietary interventions: a blended F&V mixture ("F&V Blend"), a rice-based cereal product coated with this blend ("Coated Pearl"), or the same product without the F&V mixture ("Uncoated Pearl"). The four-week study included a two-week run-in and a two-week intervention phase, each followed by a test day. Measurements included DNA damage resistance (comet assay), plasma antioxidant status (Trolox capacity and superoxide levels), microvasculature health (retinal analysis), and plasma phytochemical concentrations (colorimetric analyses or HPLC). To assess group differences, a linear mixed model was used. Fifteen polymorphic genes related to phytochemical metabolism and oxidative stress were tested using TaqMan and PCR, with outcomes analyzed via ANOVA. <b>Results</b>: The F&V Blend and Coated Pearl products increased plasma carotenoid levels versus the Uncoated Pearl product. Only the F&V Blend improved retinal dilation and DNA resistance. Surprisingly, the Uncoated Pearl product enhanced antioxidant capacity, lowered superoxide levels, and improved retinal microvasculature. Genotype effects were minimal, except for HNF1A, where wildtypes in the Uncoated Pearl group showed a higher antioxidant capacity. <b>Conclusions</b>: Fresh F&Vs were more effective than coated alternatives in improving vascular health and DNA protection.