The Increase of The Senescence in Human Lung Adenocarcinoma Cell Line A549 by Tripterygium, A Diterpenoid from Chinese Traditional Medical Herb

Since using andrographolide to increase senescence in human lung requires extremely high concentration on the andrographolide. Therefore, we want to find another molecules in TCM that also increase the senescence in human lung adenocarcinoma. I am going to measure senescence by B-Gal assay, and p21 protein abundance by western blot, and p53 activity by p53 reporter assay. Positive control is MLN4924 and negative control is DMSO/PBS. Here lists three major possible results. 1.Tripterygium can increase both p53 and p21 so that the senescence in human lung adenocarcinoma can be increased. 2 Tripterygium can only increase either p53 or p21 so that the senescence cannot be increased. 3. Triptergium can only increase either p53 or p21 that can increase the senescence. We aim to determine the efficacy of Tripterygium to increase the senescence in human lung cancer cell.