The Molecular Mechanisms by Which Knockout EDARADD Affects the Metastatic Ability of T24 Bladder Cancer Cells

Bladder cancer is a common malignant tumor in the urinary system. It has been reported that the expression of EDARADD, which is called ectodysplasin-A receptor-associated death domain, in bladder cancer is higher than in normal bladder tissue and it can impact the metastasis of bladder cancer by modulating the process of EMT. But the specific mechanism is still not clear. This study embarks on exploring the specific mechanism of how EDARADD knockout in T24 cells will affect the progress of bladder cancer. The study will test the change of Trim21 expression and Snail1 expression after EDARADD knockout in the T24 bladder cancer cells and explore whether interactions occur between EDARADD and Trim21 or between Trim21 and Snail1. With our result, we hope to show that EDARADD knockout will up-regulate the Trim21 expression, down-regulate the Snail1 expression. we also hope to show that EDARADD can interact with Trim21 and Trim21 can interact with Snail1. These results are consistent with our hypothesis.