Acute And Sub-Acute Toxicity Studies of a Poly Herbal Formulation Used for Management of Diabetes

The polyherbal solution comprising of Karela (Momordica charantia), Daruharidra (Berberis aristata), and Gudmar (Gymnema sylvestre), Amlatas (Cassis Fistula), Jamun (Syzyguium Cumin), Guduchi (Tinospora Cordifolia), Vijatsara (Pterocarpus Marsupium), Dalchini (Cinamomum Zeylenicum), Methi (Trigonella foenum graecum), Pipal (Piper Longum), Gum Acacia (Acacia Arabica) has been used for the management of Diabetes mellitus empirically. However, there are no available toxicity data for this combination of herbal formulation. Therefore, our study focused on evaluating the acute and subacute toxicity of this polyherbal formulation on Wistar rat model. Briefly the experiment was performed in two phases. The acute toxicity analysis was performed for 7days in 3 Wistar rats and sub-acute toxicity was performed on 9 Wistar rats for 28 days. For acute toxicity a single dose of 800 mg/kg and 1200mg/kg of polyherbal solution was administered to rats. While for sub-acute toxicity 2 doses variations of 100 mg and 200 mg administered orally for 28 days. Biochemical and histo-pathological analysis suggested negligible toxicity and no mortality was reported in both acute and sub-acute doses signifying non-toxicity of the poly-herbal formulation.