Antiproliferative Activity of Borassus flabellifer L. Seed Coat Extracts on Oral Carcinoma Cells

Borassus flabellifer L. is a plant in Arecaceae family, widely distributed and cultivated in tropical Asian countries. Different parts of Borassus flabellifer L., including the fruit, seeds, leaves, and roots, are used in traditional medicine due to their rich bioactive compound content. The present study aims to investigate the in vitro anti-proliferative activity of EBSC on HSC-3 cells. The extract were prepared using an ethanolic solvent, and its phytochemical composition were analyzed to identify potential bioactive compounds. The antiproliferative effects of the extract were evaluated using the Cell Counting Kit-8 (CCK-8) assay. The phytochemical analysis revealed the presence of phenolic, flavonoid, terpenoid, tannin, and alkaloid in EBSC. In CCK-8 assay, EBSC exhibited dose-dependent antiproliferative effects on HSC-3 cells. Significant inhibition of cell proliferation was observed at 750 μg/mL, comparable to doxorubicin (3 μM, p 0.001), following 24, 72, and 120 hours of treatment. EBSC shows promising potential as a natural therapeutic candidate for OSCC treatment and warrants further in vivo studies.