Participation of the TRPA1 Receptor in the Antinociceptive and Anti-Inflammatory Effect of the α, β-Amyrin Mixture in Adult Zebrafish (Danio rerio)
Inflammatory pain is a symptom associated with different human diseases, and they are treated with drugs that have several serious side effects. The triterpene mixture of α, β-amyrin (ABAM) has already been reported with antinociceptive and anti-inflammatory effects in mice. The antinociceptive potential of ABAM possibly involves the mechanisms of opioid and vanilloid receptors. This study aimed to determine the antinociceptive and anti-inflammatory potential of ABAM in adult zebrafish by modulating TRPA1. Thus, ABAM had its antinociceptive effect investigated by the nociception model in adult zebrafish induced by formalin. Furthermore, the possible neuromodulation by the TRPA1 channel was evaluated by pre-treatment with camphor. ABAM exhibited significant antinociceptive and anti-inflammatory effects in adult zebrafish at all tested doses. These effects, potentially mediated through TRPA1 modulation, were observed without signs of toxicity over a 96-hour period.
Authors
- Maria Kueirislene Amâncio Ferreira (ORCID: https://orcid.org/0000-0002-4270-8109)
- Antônio Wlisses da Silva (ORCID: https://orcid.org/0000-0002-1686-1644)
- Jesyka Macedo Guedes
- Emanuela de Lima Rebouças (ORCID: https://orcid.org/0000-0002-0572-5196)
- Amanda Maria Barros Alves (ORCID: https://orcid.org/0000-0003-3846-7311)
- Jane Eire Silva Alencar de Menezes (ORCID: https://orcid.org/0000-0001-9632-5464)
- Emmanuel Silva Marinho (ORCID: https://orcid.org/0000-0002-4774-8775)
- Maria Izabel Florindo Guedes (ORCID: https://orcid.org/0000-0002-4569-8663)
- Marnielle Rodrigues Coutinho (ORCID: https://orcid.org/0000-0001-9459-6412)
- Márcia Machado Marinho (ORCID: https://orcid.org/0000-0002-7640-2220)
- H Santosa
Institutions
- Universidade Estadual do Ceará (BR)
- State University of Vale do Acaraú (BR)
Publication Details
- Journal
- Journal of Pharmacy and Nutrition Sciences
- Published
- 2025-06-19
- DOI
- https://doi.org/10.29169/1927-5951.2025.15.06
- Primary Topic
- Pain Mechanisms and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico