A Mycorrhiza-Induced Phosphate Transporter <i>TaPT31-7A</i> Regulating Inorganic Phosphate Uptake, Arbuscular Mycorrhiza Symbiosis, and Plant Growth in Wheat
Phosphate transporters play a key role in improving crop yield. In this study, <i>TaPT31-7A</i> is a high-affinity phosphate transporter strongly induced in arbuscular-mycorrhizal (AM) wheat roots. It restores Pi uptake in yeast mutant MB192 and localizes to the plasma membrane. <i>TaPT31-7A</i> overexpression lines accumulated more shoot and root phosphorus than the wild type under both low- and high-Pi conditions. When inoculated with AM in Pi-deficient soil, these overexpression lines displayed enhanced Pi uptake, higher mycorrhization, and improved growth, ultimately increasing the spikelet number per spike, spike length, 1000-grain weight, grain length, and grain width. Transcriptome and coexpression analyses of <i>TaPT31-7A</i> OE lines and control plants showed altered expression of phosphate-starvation and AM-development genes, while docking and yeast two-hybrid assays confirmed its interaction with PP2C phosphatase <i>TaPP2C12-6A</i>. These results establish <i>TaPT31-7A</i> as a central regulator of Pi uptake, AM symbiosis, and productivity in wheat and highlight its potential for breeding phosphorus-efficient cultivars.
Authors
- Guozhang Kang (ORCID: https://orcid.org/0000-0002-0096-813X)
- Zang Zhen-chun
- Lingzhong Meng (ORCID: https://orcid.org/0000-0002-5168-5084)
- Xinxin Shangguan (ORCID: https://orcid.org/0009-0008-0273-5381)
- Yuchen Ma
- Chengwei Li (ORCID: https://orcid.org/0000-0002-3614-2276)
- Lili Li (ORCID: https://orcid.org/0009-0009-0725-2992)
- Xiaoli Li (ORCID: https://orcid.org/0000-0002-0762-6562)
- Xiaohong Zheng (ORCID: https://orcid.org/0009-0005-8570-8802)
- Lidan Zhang (ORCID: https://orcid.org/0000-0001-5230-9016)
- Yi Zhang (ORCID: https://orcid.org/0000-0003-1911-3809)
- Xueqing Wang (ORCID: https://orcid.org/0000-0001-7763-6347)
- Ying Yang (ORCID: https://orcid.org/0000-0003-4522-7703)
- Danfeng Wang (ORCID: https://orcid.org/0009-0005-4164-0447)
- Kedong Xu (ORCID: https://orcid.org/0000-0001-7399-0728)
Institutions
- Henan University of Technology (CN)
- Zhoukou Normal University (CN)
- Institute of Plant Genetics, Polish Academy of Sciences (PL)
- Institute of Crop Science (JP)
- Henan Agricultural University (CN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2025-06-19
- DOI
- https://doi.org/10.1021/acs.jafc.5c05299
- Citations
- 2
- Primary Topic
- Plant nutrient uptake and metabolism
- Type
- article
- Field-Weighted Citation Impact
- 3.50
Funders
- National Natural Science Foundation of China
- Science and Technology Department of Henan Province
- Henan Provincial Science and Technology Research Project