Establishment and Application of Loop-Mediated Isothermal Amplification Assays for Pathogens of Rice Bakanae Disease
Rice bakanae disease (RBD), a major threat in rice-cropping nations, can reduce rice yield and quality. As it is a seed-borne disease, effective seed detection is crucial. Loop-mediated isothermal amplification (LAMP) can rapidly and specifically amplify DNA at a constant temperature with high sensitivity. This research uses LAMP to develop rapid RBD pathogen detection systems. Primers were designed targeting the NRPS31 gene of Fusarium fujikuroi and conserved TEF1α sequences of Fusarium asiaticum, Fusarium proliferatum, and Fusarium andiyazi. These reactions at 60 °C for 60 min had a detection limit of 100 pg·μL−1, and LAMP proved applicable in field trials.
Authors
- Yating Zhang (ORCID: https://orcid.org/0000-0001-5745-9021)
- Yu‐Lin Song (ORCID: https://orcid.org/0000-0002-9539-588X)
- Wenjing Zheng (ORCID: https://orcid.org/0000-0001-8692-7090)
- Songhong Wei
- Han Tao (ORCID: https://orcid.org/0000-0002-2047-708X)
- Yan Wang (ORCID: https://orcid.org/0000-0003-2563-255X)
- Chenxi Liu (ORCID: https://orcid.org/0000-0001-6314-5416)
- Xinchun Liu (ORCID: https://orcid.org/0000-0002-1544-120X)
- Jian Xia (ORCID: https://orcid.org/0000-0003-3182-1461)
Institutions
- Shenyang Agricultural University (CN)
- Rice Research Institute (CN)
Publication Details
- Journal
- Agriculture
- Published
- 2025-06-19
- DOI
- https://doi.org/10.3390/agriculture15121319
- Primary Topic
- Plant Pathogens and Fungal Diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Liaoning Revitalization Talents Program
- Earmarked Fund for China Agriculture Research System