Lacticaseibacillus salsurae sp. nov. and Levilactobacillus muriae sp. nov., isolated from traditional Chinese pickle

Two novel bacteria (designated N501-2^T and N40-8-2^T) belonging to the genera <i>Lacticaseibacillus</i> and <i>Levilactobacillus</i> were isolated from traditional Chinese pickle ('Suan cai') and identified. Strain N501-2^T was phylogenetically related to the type strains of <i>Lacticaseibacillus baoqingensis</i>, <i>Lacticaseibacillus porcinae</i>, <i>Lacticaseibacillus manihotivorans</i> and <i>Lacticaseibacillus jixiensis</i>, having 98.7-99.4% 16S rRNA gene sequence similarities. Strain N40-8-2^T was phylogenetically related to the type strains of <i>Levilactobacillus fuyuanensis</i>, <i>Levilactobacillus parabrevis</i>, <i>Levilactobacillus tujiorum</i>, <i>Levilactobacillus hammesii</i>, <i>Levilactobacillus senmaizukei</i> and <i>Levilactobacillus tangyuanensis</i>, having 98.4-99.4% 16S rRNA gene sequence similarities. Strain N501-2^T had 74.0-84.2% ANI (average nucleotide identity), 20.8-28.4% dDDH (digital DNA-DNA hybridization) and 73.5-86.8% AAI (average amino acid identity) values with <i>L. manihotivorans</i> DSM 13343^T, <i>L. porcinae</i> JCM 19617^T, <i>L. jixiensis</i> N163-3-2^T and <i>L. baoqingensis</i> 47-3^T. Analyses based on whole-genome sequences indicated that strain N40-8-2^T was most closely related to the type strains of <i>L. fuyuanensis</i>, <i>L. parabrevis</i>, <i>L. tujiorum</i> and <i>L. hammesii</i>, having less than 87.4% ANI, 33.6% dDDH and 93.2% AAI values. Based upon the data obtained in the present study, two novel species, <i>Lacticaseibacillus salsurae</i> sp. nov. and <i>Levilactobacillus muriae</i> sp. nov., are proposed, and the type strains are N501-2^T (=CCTCC AB 2024124^T=JCM 37150^T=LMG 33663^T) and N40-8-2^T (=CCTCC AB 2024128^T=JCM 37001^T=LMG 33659^T), respectively.