Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri

The transcription activator FleQ is responsible for the biosynthesis of flagella and other mechanisms in several bacteria. This study reported its regulatory role on EPS production, swimming, and virulence in <i>Xanthomonas citri</i> subsp. <i>citri</i>. Knockout and over-expression experiments revealed that the response regulator FleQ positively impacted virulence and cell motility, whereas it had a negative role in EPS production. FleQ interacts directly with the <i>flgG</i> promoter and participates in the positive regulation of the expression of <i>flgG</i>. Like RpoN2, FleQ physically interacted with VemR. In a complementation analysis of an <i>rpoN2vemRfleQ</i> operon deletion mutant, the restoration of swimming motility and activation of <i>flgG</i> required the presence of the entire <i>rpoN2vemRfleQ</i> operon. Together, our data suggest that FleQ plays multiple regulatory roles, especially activating the expression of <i>flgG</i> jointly with RopN2 and VemR in <i>X. citri</i> subsp. <i>citri</i>.