Magnetically Regulated Multiparameter Detection System for Magnetically Labeled Biomolecules

Magnetically labeled biomolecule detection technology is playing an increasingly important role in disease diagnosis, drug development and other fields. Biomolecules labeled with magnetic particles can be regulated to move under a magnetic field, and the motion signals are feasible to be acquired using quartz crystal microbalance (QCM). By analyzing the motion signals of biomolecules, various property parameters that determine the motion characteristics can be detected. Based on this mechanism, we developed a magnetically labeled biomolecule multi-parameter detection instrument system. To accommodate the detection of various molecular parameters, the design of the instrument system provides ample flexibility for application: the magnetic field excitation source in the system can generate arbitrary waveform excitations and the sensor QCM is free of biochemical decoration. To enhance the detection sensitivity and resolution of the system, the QCM output signal is demodulated first and only the molecular motion signal is collected for analysis, with digital signal processing and analysis being completed by a computer. Test results indicated that the system has achieved the detection of parameters such as the magnetic bead concentration (ng/mL level), the hydrodynamic dimension of magnetically labeled biomolecules (nm level). This instrument system functions as an open platform for biomolecule multi-parameter detection, capable of completing more magnetically labeled molecule parameter detections and biochemical dynamic process monitoring. It represents a new instrument technology for biochemical molecule detection.