Adapting to UV: Integrative Genomic and Structural Analysis in Bacteria from Chilean Extreme Environments

Extremophilic bacteria from extreme environments, such as the Atacama Desert, Salar de Huasco, and Antarctica, exhibit adaptations to intense UV radiation. In this study, we investigated the genomic and structural mechanisms underlying UV resistance in three bacterial isolates identified as <i>Bacillus velezensis</i> PQ169, <i>Pseudoalteromonas</i> sp. AMH3-8, and <i>Rugamonas violacea</i> T1-13. Through integrative genomic analyses, we identified key genes involved in DNA-repair systems, pigment production, and spore formation. Phylogenetic analyses of aminoacidic sequences of the nucleotide excision repair (NER) system revealed conserved evolutionary patterns, indicating their essential role across diverse bacterial taxa. Structural modeling of photolyases from <i>Pseudoalteromonas</i> sp. AMH3-8 and <i>R. violacea</i> T1-13 provided further insights into protein function and interactions critical for DNA repair and UV resistance. Additionally, the presence of a complete violacein operon in <i>R. violacea</i> T1-13 underscores pigment biosynthesis as a crucial protective mechanism. In <i>B. velezensis</i> PQ169, we identified the complete set of genes responsible for sporulation, suggesting that sporulation may represent a key protective strategy employed by this bacterium in response to environmental stress. Our comprehensive approach underscores the complexity and diversity of microbial adaptations to UV stress, offering potential biotechnological applications and advancing our understanding of microbial resilience in extreme conditions.