Optimization Studies on Reaction Parameters for Silver Nanoparticle Synthesis in Medicinal Plants

Optimization process plays a vital role in nanotechnological research by enhancing the production of desired nanoparticles with controlled shape and size within a specific time duration. Different reaction parameters such as pH, volume, temperature, time etc. can be optimized which helps in effective synthesis rate. In the present study, optimization was carried out for silver nanoparticles synthesized using aqueous extracts from dried leaf samples ofLawsonia inermis L., Piper betle L. and Mangifera indica L. Various reaction conditions were optimized such as concentration of silver nitrate (0.5 mM - 2.5mM), ratio of extract: silver nitrate volume (1:10 – 5:10), pH (3 – 11), temperature (-20˚C - 100˚C) and reaction time (30 minutes – 36 hours). Based on the optimization studies, all three samples were observed to exhibit similar optimum conditions of 1mM silver nitrate concentration, 1:10 volume ratio, pH of 7, 37˚C temperature and time duration of 24 hours.