Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR

This study shows the potential of the bovine bead-based multiplex immunoassay for measuring IL-10, IL-17, TNF-α, and IFN-γ concentrations in reindeer. The qPCR is suitable for measuring gene expression of these cytokines and IL-8. These methods may be used to characterise immune responses in reindeer, but further testing and validation are warranted.

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Publication Details

Journal
Acta veterinaria Scandinavica
Published
2025-06-18
DOI
https://doi.org/10.1186/s13028-025-00819-4
Primary Topic
Animal Disease Management and Epidemiology
Type
article
Field-Weighted Citation Impact
0.00

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article

Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR

Carlos G. das Neves, Kari Lybeck, Morten Tryland, Ingebjørg Helena Nymo et al.
Acta veterinaria Scandinavica
Animal Disease Management and Epidemiology
article

Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR

Carlos G. das Neves, Kari Lybeck, Morten Tryland, Ingebjørg Helena Nymo, Siv Klevar, Hege Lund, Shokouh Makvandi‐Nejad, Tuva Løken Frøvoll
article en

Abstract

This study shows the potential of the bovine bead-based multiplex immunoassay for measuring IL-10, IL-17, TNF-α, and IFN-γ concentrations in reindeer. The qPCR is suitable for measuring gene expression of these cytokines and IL-8. These methods may be used to characterise immune responses in reindeer, but further testing and validation are warranted.

Acta veterinaria ScandinavicaVol. 67(1)
Norwegian Food Safety Authority (NO), University of Inland Norway (NO), Norwegian Veterinary Institute (NO), Norwegian University of Life Sciences (NO), UiT The Arctic University of Norway (NO)
Universitetet i Tromsø, Norges Forskningsråd
Climate action
Openalex Percentile: Top 82%
Animal Disease Management and Epidemiology
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Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR — Carlos G. das Neves, Kari Lybeck, et al. · Acta veterinaria Scandinavica (2025) | TGRS Research Map | TGRS