Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice
Introducing additional genetic modifications or environmental stimuli accelerates early pancreatic carcinogenesis in the p48<sup>Cre/+</sup>; LSL-Kras<sup>G12D/+</sup> (KC) mouse model. Here, we present a protocol employing adeno-associated virus (AAV)-mediated gene delivery specifically in the pancreas to facilitate the progression from acinar cells through acinar-to-ductal metaplasia (ADM) to pancreatic intraepithelial neoplasia (PanIN). We describe steps for AAV production, preparing KC mice, intra-pancreatic AAV injection, and analysis. This protocol allows the investigation of early events of pancreatic carcinogenesis in a spatially and temporally controlled manner. For complete details on the use and execution of this protocol, please refer to Li et al.<sup>1</sup>.
Authors
- Hongzhen Li (ORCID: https://orcid.org/0000-0001-6393-3009)
- Shanshan Shen (ORCID: https://orcid.org/0000-0001-9967-7764)
- Zhao Shi
Institutions
- Heidelberg University (DE)
- Nanjing Drum Tower Hospital (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-06-18
- DOI
- https://doi.org/10.1016/j.xpro.2025.103907
- Primary Topic
- Virus-based gene therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Jiangsu Commission of Health
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- Natural Science Foundation of Jiangsu Province
- Jiangsu Provincial Commission of Health and Family Planning