A benchmark dataset for validating <i>FKS1</i> mutations in <i>Candida auris</i>
Echinocandins are the recommended first-line treatment for invasive infections caused by <i>Candida auri</i>s, a multi-drug-resistant yeast that has emerged in healthcare facilities globally. Increasing instances of echinocandin-resistant cases highlight the need for rapid detection and response. We developed a benchmark dataset comprising 100 <i>C. auris</i> echinocandin-resistant and -susceptible isolates to demonstrate the utility of the bioinformatics tool, MycoSNP-nf, for detecting echinocandin resistance-related <i>FKS1</i> mutations and to assess their concordance with antifungal susceptibility testing results. This benchmark may help validate MycoSNP-nf and other bioinformatics tools aimed at detecting these mechanisms using whole-genome sequencing data.
Authors
- Lalitha Gade (ORCID: https://orcid.org/0000-0002-9264-9952)
- Nancy A. Chow (ORCID: https://orcid.org/0000-0002-3777-9500)
- Zachary B. Mudge (ORCID: https://orcid.org/0009-0007-0354-5531)
- Amanda Ribeiro dos Santos (ORCID: https://orcid.org/0000-0002-5577-4498)
- Kaitlin Forsberg (ORCID: https://orcid.org/0000-0002-5779-2446)
- Shawn R. Lockhart (ORCID: https://orcid.org/0000-0002-4383-5994)
- Meghan Lyman (ORCID: https://orcid.org/0000-0001-7925-2232)
- D. Joseph Sexton (ORCID: https://orcid.org/0000-0003-3099-7334)
- Luisa F. López (ORCID: https://orcid.org/0000-0001-7994-6621)
- Elizabeth Misas (ORCID: https://orcid.org/0000-0001-6243-7716)
- Malavika Rajeev (ORCID: https://orcid.org/0000-0001-6030-4995)
- Lindsay A. Parnell (ORCID: https://orcid.org/0000-0001-5598-6485)
- Anastasia P. Litvintseva (ORCID: https://orcid.org/0000-0003-1086-8041)
Institutions
- Centers for Disease Control and Prevention (US)
- United States Department of Health and Human Services (US)
- The Centers (US)
- Centers for Disease Control and Prevention (KE)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-06-18
- DOI
- https://doi.org/10.1128/spectrum.03147-24
- Citations
- 3
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 4.19