Hyperspectral imaging system with switchable spontaneous Raman spectroscopy and broadband CARS

We present a novel, switchable hyperspectral imaging system that integrates both broadband Coherent Anti-Stokes Raman Scattering (bCARS) and confocal Spontaneous Raman (SR) spectroscopy within a single optical platform. The system is designed to enable full-spectrum acquisition across a broad wavenumber range (400 to 4000cm⁻¹) for both modalities. It facilitates direct, pixel-to-pixel comparison of Raman and bCARS modalities under identical experimental conditions. Combining both modalities allows the speed of bCARS to be married with the spectral fidelity of SR for chemically specific hyperspectral imaging. This hybrid approach provides a flexible and powerful tool for high-throughput hyperspectral Raman imaging with applications in biomedical diagnostics, materials analysis, and beyond.