ATPase level in Iraqi Diabetic Patients with and without Diabetic Kidney Disease

Complications of diabetes have become one of the main problems that diabetics suffer from, especially during long periods. One of these complications is diabetic nephropathy, as both glomeruli and tubules are exposed to fibrosis due to diabetes. Recent years have witnessed great interest by researchers regarding diabetic nephropathy. This study aimed to determine the level of ATPase and some of the relevant biochemical factors in patients with diabetes and diabetic nephropathy compared with healthy controls. The study included 120 male and female (60 male and 60 female) ranging in age (30-65) years old. Ninety patients with type 2 diabetes are subdivided into three groups on the basis of ACR criteria including normoalbuminuria, microalbuminuria, macroalbuminuria (30 patients for each group). A control group of 30 healthy people was included. This study was conducted at Baghdad Teaching Hospital / Medical City and Al-Yarmouk Teaching Hospital during the period between December 2021 and May 2022 ATPase levels were determined using the ELISA technique, and HbA1c was assessed using the I- chroma device. ATPase levels were found to be significantly increased in patient groups than in healthy control. On the basis of the obtained results in this study, we can be concluded that Na-K ATPase can be applied as a reliable prognosticated factor between disease and normal cases. Furthermore, area under the curve (ROC) analysis data revealed that ATPase was found to be a perfect marker for follow-up diabetic nephropathy disease.