Identification of a gene conferring broad-spectrum orthotospovirus resistance in Solanaceae
Linkage drag can hinder the integration of resistance genes from wild crop relatives into breeding programs. We used a chromosome-scale <i>Nicotiana alata</i> genome assembly and a segregating population exceeding 160,000 plants to dissect the complex genetic architecture and overcome the tight linkage between resistance and deleterious loci to produce plants free from linkage drag. We cloned <i>N. alata RTSW</i>, encoding an immune receptor that confers broad-spectrum resistance to orthotospoviruses through the interaction of its carboxyl-terminal domain with an orthotospovirus-encoded protein. Notably, despite recognizing the same avirulence factor, <i>RTSW</i> genes from <i>N. alata</i> and <i>Sw-5b</i> from <i>Solanum peruvianum</i> have evolved independently of adjacent nonorthologous ancestral loci. Our work illustrates the potential of wild relative genomes as resources from which to precisely introduce disease resistance into cultivated crops.
Authors
- Jiongjiong Chen
- Xiaorong Tao (ORCID: https://orcid.org/0000-0003-1259-366X)
- Changjun Huang (ORCID: https://orcid.org/0000-0003-3131-8743)
- Weishu Fan (ORCID: https://orcid.org/0000-0003-3688-5325)
- Zhijun Tong (ORCID: https://orcid.org/0000-0001-9892-0443)
- Polina Novikova (ORCID: https://orcid.org/0000-0002-4552-4575)
- Xueyi Sui (ORCID: https://orcid.org/0000-0002-3981-6109)
- Chongkun Zuo (ORCID: https://orcid.org/0009-0001-9904-0912)
- Hanhui Kuang (ORCID: https://orcid.org/0000-0003-4870-0578)
- Yuan Cheng (ORCID: https://orcid.org/0000-0003-2582-4041)
- Haiqin Yu
- Jie Wang (ORCID: https://orcid.org/0000-0002-3895-8527)
- Min Zhu
- Yong Liu (ORCID: https://orcid.org/0000-0002-7793-7606)
- Yi Xu (ORCID: https://orcid.org/0000-0002-1913-4530)
- Jianmin Zeng (ORCID: https://orcid.org/0000-0002-3300-9779)
Institutions
- Nanjing Agricultural University (CN)
- Chinese Academy of Sciences (CN)
- Huazhong Agricultural University (CN)
- China Tobacco (CN)
- Tobacco Research Institute (CN)
- Institute of Genetics and Developmental Biology (CN)
- Yunnan Academy of Agricultural Sciences (CN)
- Max Planck Institute for Plant Breeding Research (DE)
Publication Details
- Journal
- Science Advances
- Published
- 2025-06-18
- DOI
- https://doi.org/10.1126/sciadv.adw4333
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00