Sample Preparation Methods Focusing on Soy Biotechnological Samples: Towards Greener Application

Tissue and cell culture techniques have become pivotal in plant biotechnology, particularly for the improvement and mass propagation of economically important species such as soybean (Glycine max). Within this context, somatic embryogenesis has emerged as a powerful tool. Micronutrients like Fe, Mn, Mo, and Zn play essential roles in cellular metabolism and can serve as indicators of tissue growth and development. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) is widely recognized for its sensitivity in multielement analysis at trace levels; however, sample preparation remains a critical and often resource-intensive step.