SERUM BIOCHEMICAL PARAMETERS DURING DIFFERENT STAGES OF PREGNANCY IN MURRAH BUFFALOES

The most significant and productive milk producer among Indian buffalo breeds is the Murrah. Determining reference values for the serum biochemical markers of Murrah buffaloes at different stages of pregnancy was the aim of the current study. This study included 28 Murrah buffaloes from the Indore district of Madhya Pradesh, India. They have been separated into 4 groups: Group I was non-pregnant, Group 2 was in “the 1st trimester of pregnancy, Group 3 was in the 2nd trimester of pregnancy, and Group 4 was in the 3rd trimester of pregnancy” till parturition. Every group had 7 animals. Blood samples were tested for serum biochemical measures, including total albumin, BUN (blood urea nitrogen), AST (aspartate aminotransferase), glucose, uric acid, globulin, HDL (higher density lipoprotein) cholesterol, A/G (albumin/globulin) ratio, LDL (lower density lipoprotein) cholesterol, creatinine, total cholesterol, ALT (alanine-aminotransferase), triglycerides, ALP (alkaline phosphatase), andprotein. Late-pregnancy serum albumin levels in comparison to mid-pregnancy and non-pregnant animals; HDL cholesterol in early-, mid-, and late-pregnancy in animals as well as non-pregnant, mid-, as well as late-pregnant animals; ALT as well as AST levels in late pregnancy in comparison to other cohorts; glucose levels in pregnant animals as opposed to those that are not; Urea levels in non-pregnant, early, middle, and late-pregnant animals; BUN between early-, middle-, and late-stage pregnancy and non-pregnant animals; early-pregnant with late pregnant animals-all displayed a greatly significant variance (P<0.01). Serum “levels of total protein in the middle along with late phase of pregnancy has been significantly (P<0.05) different from those of the non-pregnant” animal, total cholesterol in the 3rd trimester of pregnancy was different from other groups, and only uric acid was different between the non-pregnant and postpartum animals. The levels of globulin, LDL cholesterol, A/G ratio, ALP, triglycerides, as well as creatinine did not significantly differ (P>0.05) across the groups. The current research concluded by establishing reference values that serve as a helpful guide for evaluating blood biochemical markers in Murrah buffaloes at various stages of pregnancy.