Development and implementation of a TaqMan triplex real-time PCR assay for concurrent detection of pseudorabies virus, porcine teschovirus 1, and Streptococcus suis 2
The triplex real-time PCR method developed in this study effectively distinguishes PRV, PTV1, and SS2 simultaneously, serving as a valuable diagnostic tool. This method is anticipated to play a crucial role in preventing and controlling infectious disease spread and supporting epidemiological investigations.
Authors
- Zhiqiang Hu (ORCID: https://orcid.org/0000-0001-8854-3410)
- Wei Liu (ORCID: https://orcid.org/0000-0001-9037-8792)
- Li Juan Wu (ORCID: https://orcid.org/0000-0002-5468-2487)
- Zheng Yan
- Shifang Ren (ORCID: https://orcid.org/0000-0001-8931-9293)
- Yang Chen (ORCID: https://orcid.org/0000-0002-4722-279X)
- Lulu Li (ORCID: https://orcid.org/0000-0002-5559-8236)
- Wei-Sheng Wu (ORCID: https://orcid.org/0000-0002-9919-9579)
- Xiaowen Li (ORCID: https://orcid.org/0000-0001-9776-5680)
- Deshan Yu
- Ranran Lai
Institutions
- Animal Husbandry & Veterinary (IN)
- North West Agriculture and Forestry University (CN)
- Xichang University (CN)
- New Hope Liuhe (China) (CN)
Publication Details
- Journal
- Frontiers in Veterinary Science
- Published
- 2025-06-18
- DOI
- https://doi.org/10.3389/fvets.2025.1589175
- Citations
- 1
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.19