POTENTIAL OF THE Spatholobus littoralis AS AN ANTIBACTERIAL AND PREDICTION OF THE MECHANISM OF ACTIVITY AGAINST PBP3 RECEPTOR MODEL OF Staphylococcus aureus

Spatholobus littoralis is a typical plant of Kalimantan, which is ethnopharmacologically used in herbal medicine. The purpose of this study was to test the activity of S. littoralis root extract against Staphylococcus aureus bacteria in vitro and to predict the mechanism of its interaction with Staphylococcus aureus penicillin-binding protein-3 (PBP-3) in silico. Extraction of S. littoralis using ethanol solvent, and continued with sequential fractionation using n-hexane, chloroform, and ethyl acetate. Antibacterial activity test against Staphylococcus aureus was carried out by agar diffusion using the Kirby-Bauer test method. The prediction of activity mechanism against the 3VSL receptor using ligands from compounds that have been found in the genus Spatholobus. Extracts and fractions of S. littoralis root have antibacterial activity against Staphylococcus aureus. Several compounds were found in the genus Spatholobus. It showed stronger binding energy than natural ligands. The compound 6-methoxyeriodictyol shows the most stable binding energy to the 3VSL receptor; the binding site of the compound is at the amino acid residues Tyr636, Lys395, and Asn 450. Thus, the roots of the S. littoralis plant have the potential to be developed as a natural antibacterial.