Housing matters: Experimental variables shaping metabolism in obese mice
Diet-induced obesity in mice is an important model for investigating host-diet interactions as well as dietary and pharmacological treatments of metabolic diseases. Experimental reproducibility is, however, a recurrent challenge. To determine key controllable experimental drivers of mouse metabolism, we distributed 338C57BL/6JBomTac mice (males and females) into six research units across two countries, divided them into a variety of housing conditions (i.e., diets, cage types, temperatures, group-housing vs. single-housing) and kept 26 reference mice at the vendor. We applied linear mixed models to rank the influence of each variable on metabolic phenotype (i.e., body weight gain, glucose intolerance, liver, and visceral adipose tissue weight). Group-housing was the most potent driver of metabolic dysfunction apart from sex and diet. Accordingly, single-housed mice exhibited reduced weight gain (∼50%), increased energy expenditure, and diminished respiratory exchange ratio concomitant with improved glucose tolerance (∼20%) compared to their group-housed counterparts. Our results may aid in clarifying the impact of experimental design and promote rational, transparent reporting to increase reproducibility.
Authors
- Otto Kalliokoski (ORCID: https://orcid.org/0000-0001-6531-3487)
- Béatrice S.-Y. Choi (ORCID: https://orcid.org/0000-0001-8160-9448)
- Lene Secher Myrmel (ORCID: https://orcid.org/0000-0002-8260-1648)
- Karsten Kristiansen (ORCID: https://orcid.org/0000-0002-6024-0917)
- Lise Madsen (ORCID: https://orcid.org/0000-0003-4468-1947)
- Jonas T. Treebak (ORCID: https://orcid.org/0000-0003-1488-7012)
- David M. Kristensen (ORCID: https://orcid.org/0000-0003-0657-1632)
- Even Fjære (ORCID: https://orcid.org/0000-0003-0326-1233)
- Benjamin A. H. Jensen (ORCID: https://orcid.org/0000-0001-6991-0828)
- Axel Kornerup Hansen (ORCID: https://orcid.org/0000-0003-1575-2507)
- Asker Brejnrod (ORCID: https://orcid.org/0000-0002-1327-2051)
- Xiao Liang (ORCID: https://orcid.org/0000-0001-5476-8304)
- Jacob Bak Holm (ORCID: https://orcid.org/0000-0003-1756-0875)
- Ida Larsen
- Helle Sørensen (ORCID: https://orcid.org/0000-0001-5273-6093)
- Manimozhiyan Arumugam (ORCID: https://orcid.org/0000-0002-0886-9101)
- Morten Dall (ORCID: https://orcid.org/0000-0002-8577-3162)
- Niels Banhos Danneskiold‐Samsøe (ORCID: https://orcid.org/0000-0002-2889-5630)
- Zhongkui Xia (ORCID: https://orcid.org/0000-0003-3786-9394)
- Marie-Louise Allingbjerg
- Janne Koch
Institutions
- BGI Group (China) (CN)
- University of Copenhagen (DK)
- Novo Nordisk Foundation (DK)
- Norwegian Institute of Marine Research (NO)
- University of Bergen (NO)
Publication Details
- Journal
- Molecular Metabolism
- Published
- 2025-06-18
- DOI
- https://doi.org/10.1016/j.molmet.2025.102190
- Citations
- 2
- Primary Topic
- Adipose Tissue and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 2.33
Funders
- Lundbeckfonden
- Novo Nordisk
- Danmarks Frie Forskningsfond
- Novo Nordisk Fonden
- Novo Nordisk Foundation Center for Basic Metabolic Research
- H. Lundbeck A/S
- Centro de Investigação em Biomedicina