Integrated Phylogenomics and Expression Profiling of the TRM Gene Family in Brassica napus Reveals Their Role in Development and Stress Tolerance

The <i>TRM</i> (<i>TONNEAU1 Recruiting Motif</i>) gene family plays a crucial role in multiple biological processes, including microtubule organization, cell division regulation, fruit morphogenesis, stress adaptation, and growth and development. To delve deeper into the potential functions of <i>BnaTRMs</i> in <i>Brassica napus</i>, this study employed bioinformatics methods to systematically identify and analyze the <i>TRM</i> family genes in <i>Brassica napus</i> (Westar). Using the model plant <i>Arabidopsis thaliana</i> as a reference and based on six conserved motifs, 100 <i>TRM</i> members were first identified in <i>Brassica napus</i>. These genes are widely distributed across 19 chromosomes, and most exhibit nuclear localization characteristics. Through gene collinearity analysis among <i>Brassica napus</i>, <i>Arabidopsis thaliana</i>, <i>Glycine max</i>, <i>Oryza sativa</i>, and <i>Zea mays</i>, we speculate that <i>Brassica napus</i> and <i>Glycine max</i> may share a similar evolutionary history. Analysis of cis-acting elements in the 2000 bp upstream region of <i>TRM</i> gene promoters revealed numerous elements related to abiotic stress response and hormone regulation. Furthermore, qRT-PCR data supported these findings, indicating that multiple <i>TRM</i> genes actively participate in the growth and development process and abiotic stress tolerance of <i>Brassica napus</i>. In summary, <i>BnaTRMs</i> exhibit significant functions in stress adaptation, growth, and development. This study not only enhances our understanding of the functions of the <i>TRM</i> gene family but also provides new perspectives and strategies for further exploring their regulatory mechanisms and potential applications.