Enzymatic biofuel cell on flexible nanoporous gold electrodes
A glucose/O<sub>2</sub>-based biofuel cell employing nanoporous gold electrodes (NPG) supported by Kapton® was prepared. The anode was prepared by drop-casting a solution of Crassicarpon hotsonii cellobiose dehydrogenase (ChCDH) and an Os complex-based polymer and the cathodes by covalent immobilization of Magnaporthe oryzae bilirubin oxidase (MoBOD) on 3-mercaptopropionic acid (MPA) self-assembled monolayer (SAM). Both electrodes were coated with poly(2-methacryloyloxyethyl phosphorylcholine-co-glycidyl methacrylate) (MPC) to reduce biofouling. The anode had a J<sub>max</sub> of 172 ± 10 μA cm<sup>-2</sup> and a K<sub>Mapp</sub> of 19 ± 3 mM in phosphate buffer saline, with a linear detection range from 1 to 5 mM and a sensitivity of 9.4 ± 0.3 μA cm<sup>-2</sup> mM<sup>-1</sup>. In artificial plasma, the response was saturated at 3 mM, with J<sub>max</sub> of 6.8 ± 10 μA cm<sup>-2</sup>, K<sub>Mapp</sub> of 1 mM and a linear detection range from 1 to 5 mM. The cathode had a J<sub>max</sub> of 103 μA cm<sup>2</sup> and retained 80 % of its response after 18 h of continuous measurement in phosphate buffered saline, while in artificial plasma, the stability was significantly reduced with a half-life of 1 h under continuous operation. The power outputs in PBS and artificial serum were 4.4 and 1.0 μWcm<sup>-2</sup>, respectively.
Authors
- Thomas M. B. Reichhart (ORCID: https://orcid.org/0000-0002-0538-108X)
- Edmond Magner (ORCID: https://orcid.org/0000-0003-2042-556X)
- António L. De Lacey (ORCID: https://orcid.org/0000-0002-9347-0452)
- Sébastien Gounel (ORCID: https://orcid.org/0000-0002-7602-9657)
- Tanushree Mandal
- Wolfgang W. Schuhmann (ORCID: https://orcid.org/0000-0003-2916-5223)
- Nicolas Mano (ORCID: https://orcid.org/0000-0001-7084-9323)
- Roland Ludwig (ORCID: https://orcid.org/0000-0002-5058-5874)
- Andrés Felipe Quintero-Jaime (ORCID: https://orcid.org/0000-0003-2447-9872)
- C. Schulz (ORCID: https://orcid.org/0000-0002-0833-1520)
- Julia Álvarez-Malmagro (ORCID: https://orcid.org/0000-0001-5563-2249)
- Alfons K. G. Felice (ORCID: https://orcid.org/0000-0002-3842-1471)
- Dónal Leech (ORCID: https://orcid.org/0000-0001-7844-1306)
- Anna Lielpētere (ORCID: https://orcid.org/0000-0002-4727-0935)
- Marcos Pita (ORCID: https://orcid.org/0000-0002-6714-3669)
- Denise Demurtas (ORCID: https://orcid.org/0000-0003-0525-6083)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université de Bordeaux (FR)
- Ollscoil na Gaillimhe – University of Galway (IE)
- University of Limerick (IE)
- Instituto de Catálisis y Petroleoquímica (ES)
- Centre de recherche Paul Pascal (FR)
- Ruhr University Bochum (DE)
- BOKU University (AT)
Publication Details
- Journal
- Bioelectrochemistry
- Published
- 2025-06-17
- DOI
- https://doi.org/10.1016/j.bioelechem.2025.109034
- Primary Topic
- Electrochemical sensors and biosensors
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Commission